Publications
Articles, Reviews, and PREPRINTS

47. Abeyrathna SS, Abeyrathna NS, Basak P, Irvine GW, Zhang L, Meloni G* “Plastic recognition and electrogenic uniport translocation of 1st-, 2nd- and 3rd-row transition and post transition metals by transmembrane P1B-2-type ATPase pumps”, Chemical Science, in press, (2023) (posted on ChemRxiv, DOI: 10.26434/chemrxiv-2022-q7rcn). 

46. Ong WSY, Ji K, Pathiranage V, Maydew C, Baek K, Villones RLE, Meloni G, Walker A, Dodani SC “Rational design of the β-bulge gate in a green fluorescent protein accelerates the kinetics of sulfate sensing.” Angewandte Chemie International Edition, Apr 14; e202302304, (2023) doi:10.1002/anie.202302304. Online ahead of print. PMID: 37059690.

45. Peng W, Maydew CC, Kam H, Lynd JK, Tutol JN, Phelps SM, Abeyrathna S, Meloni G, Dodani SC. Discovery of a monomeric green fluorescent protein sensor for chloride by structure-guided bioinformatics. Chemical Science Oct 6;13(43):12659-12672, (2022) doi: 10.1039/d2sc03903f. PMID: 36519056; PMCID: PMC9645410.

44. Bulathge AW, Villones RLE, Herbert FC, Gassensmith JJ, Meloni G*. Comparative cisplatin reactivity towards human Zn7-metallothionein-2 and MTF-1 zinc fingers: potential implications in anticancer drug resistance. Metallomics. Sep 15;14(9):mfac061, (2022) doi: 10.1093/mtomcs/mfac061. PMID: 36026541; PMCID: PMC9477119.

43. Salustros N, Grønberg C, Abeyrathna NS, Lyu P, Orädd F, Wang K, Andersson M, Meloni G, Gourdon P. Structural basis of ion uptake in copper-transporting P1B-type ATPases. Nature Communications Aug 31;13(1):5121, (2022) doi: 10.1038/s41467-022-32751-w. PMID: 36045128; PMCID: PMC9433437.

42. Li P, Hendricks AL, Wang Y, Villones RLE, Lindkvist-Petersson K, Meloni G, Cowan JA, Wang K, Gourdon P. Structures of Atm1 provide insight into [2Fe-2S] cluster export from mitochondria. Nature Communications Jul 27;13(1):4339, (2022) doi:10.1038/s41467-022-32006-8. PMID: 35896548; PMCID: PMC9329353.

41. Li P, Nayeri N, Górecki K, Becares ER, Wang K, Mahato DR, Andersson M, Abeyrathna SS, Lindkvist-Petersson K, Meloni G, Missel JW, Gourdon P. PcoB is a defense outer membrane protein that facilitates cellular uptake of copper. Protein Science Jul;31(7):e4364, (2022) doi: 10.1002/pro.4364. PMID: 35762724; PMCID:PMC9210255.

40. Mehlenbacher MR, Elsiesy R, Lakha R, Villones RLE, Orman M, Vizcarra CL, Meloni G*, Wilcox DE*, Austin RN*. Metal binding and interdomain thermodynamics of mammalian metallothionein-3: enthalpically favoured Cu+ supplants entropically favoured Zn2+ to form Cu4+ clusters under physiological conditions. Chemical Science Apr 4;13(18):5289-5304, (2022) doi: 10.1039/d2sc00676f. PMID: 35655557; PMCID: PMC9093145.

39. Calvo JS, Villones RLE, York NJ, Stefaniak E, Hamilton GE, Stelling AL, Bal W, Pierce BS, Meloni G*. Evidence for a Long-Lived, Cu-Coupled and Oxygen-Inert Disulfide Radical Anion in the Assembly of Metallothionein-3 Cu(I)4-Thiolate Cluster. Journal of the American Chemical Society. Jan 19;144(2):709-722, (2022) doi: 10.1021/jacs.1c03984. Epub 2022 Jan 5. PMID: 34985880. (Highlighted in JACS Spotlight J. Am. Chem. Soc, 2022 144, 2, 625).

38. Grønberg C, Hu Q, Mahato DR, Longhin E, Salustros N, Duelli A, Lyu P, Bågenholm V, Eriksson J, Rao KU, Henderson DI, Meloni G, Andersson M, Croll T, Godaly G, Wang K, Gourdon P. Structure and ion-release mechanism of PIB-4-type ATPases. Elife.  Dec 24;10:e73124. (2021) doi: 10.7554/eLife.73124. PMID: 34951590; PMCID: PMC8880997.

37. Herbert FC, Abeyrathna SS, Abeyrathna NS, Wijesundara YH, Brohlin OR, Carraro F, Amenitsch H, Falcaro P, Luzuriaga MA, Durand-Silva A, Diwakara SD, Smaldone RA, Meloni G*, Gassensmith JJ*. Stabilization of supramolecular membrane protein-lipid bilayer assemblies through immobilization in a crystalline exoskeleton. Nature Communications Apr 13;12(1):2202. (2021) doi: 10.1038/s41467-021-22285-y. PMID: 33850135. Read the news article.

36. Tutol, J. N.; Lee, J.; Chi, H.; Faizuddin, F.‡; Abeyrathna, S. S.; Zhou, Q.; Morcos, F.; Meloni, G.; Dodani, S. C.* “A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride.” Chemical Science, Mar 17; 12(15):5655-5663. (2021); ChemRxiv. Preprint. [DOI: 10.26434/chemrxiv.13028192.v1].

35. M.J. Gallenito, T.S. Qasim, J.N. Tutol, V. Prakash, S.C. Dodani, G. Meloni* “A recombinant platform to characterize the role of transmembrane protein hTMEM205 in Pt(II)-drug resistance and extrusion”, Metallomics, 12, 1542-1554, (2020). PMID: 32789331

34. J.S. Calvo, N. Mulpuri, A. Dao, N. Qazi, G. Meloni* “Membrane insertion exacerbates the α-Synuclein-Cu(II) dopamine oxidase activity: metallothionein-3 targets and silences all α-Synuclein-Cu(II) complexes”, Free Radical Biology & Medicine, 158, 149-161, (2020). PMID: 32712192

33. N.S. Abeyrathna, S.S. Abeyrathna, M.T. Morgan, C.J. Fahrni, G. Meloni* “Transmembrane Cu(I) P-type ATPase Pumps Are Electrogenic Uniporters”, Dalton Transactions, in press (2020) (featured on Journal cover, New Talent: America Issue; selected as Hot Article by Dalton Trans.). PMID: 32469032

32. F. Arnold, M. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C. Hutter, Eike Peters, Jörn Piel, L. Hürlimann, G. Meloni, O. Medalia, M. Seeger “The ABC exporter IrtAB imports and reduces mycobacterial siderophores”, Nature, 580, 413-417 (2020)
​(featured on Nature Microbiol. Reviews Commentary, Science Daily, Phys.org, Innovation reports, Bioengineer, GIT Laborportal, VBIO, Jura Forum, UZH News). PMID: 32469032

31. A. Santoro, J.S. Calvo, M.D. Paris-Diaz, A. Krezel, G. Meloni, P. Faller “The glutathione/metallothionein system challenges the design of efficient O2-activating Cu-complexes”, Angewandte Chemie International Edition, 59, 7830-7835 (2020). PMID: 3246903230. 

30. S.D. Boyd, M.S. Ullrich, J.S. Calvo, F. Behnia, G. Meloni, D.D. Winkler “Mutations in Superoxide Dismutase 1 (Sod1) Linked to Familial Amyotrophic Lateral Sclerosis Can Disrupt High-Affinity Zinc-Binding Promoted by the Copper Chaperone for Sod1 (Ccs)”, Molecules, 25, 1086 (2020). PMID: 32121118

29. S.S. Abeyrathna, N.S. Abeyrathna, N.K. Thai, P. Sarkar, S. D'Arcy, G. Meloni* “IroT/MavN Is a Legionella Transmembrane Fe(II) Transporter: Metal Selectivity and Translocation Kinetics Revealed by in Vitro Real-Time Transport”, Biochemistry, 58, 4337-4342, (2019). PMID: 31589416     
                                                                   
28. M.J. Gallenito, G.W. Irvine, L. Zhang, G. Meloni* “Coordination promiscuity guarantees metal substrate selection in transmembrane primary-active Zn2+ pumps”, Chemical Communications, 55, 10844-10847 (2019) (featured on Journal Issue Cover). PMID:31589416      
                                                                             
27. N. Paranawithana, A. Martins, V. Clavijo-Jordan, P. Zhao, S. Chirayil, G. Meloni, A.D. Sherry “A responsive MRI contrast agent for detection of excess copper(II) in the liver in vivo”, Journal of the American Chemical Society, 141, 11009-11018 (2019). PMID: 31268706

26. M.T. Morgan, D. Bourassa, S. Harankhedkar, A.M. McCallum, S. Zlatic, J. Calvo, G. Meloni, V. Faundez, C. Fahrni, “Ratiometric two-photon microscopy reveals attomolar copper buffering in normal and Menkes mutant cells”, Proceedings of The National Academy of Sciences, 116, 12167-12172 (2019). PMID: 31160463

25. S.D. Boyd, J.S. Calvo, L. Liu, M.S. Ullrich, A. Skopp, G. Meloni, D.D. Winkler, “The yeast copper chaperone for copper-zinc superoxide dismutase (CCS1) is a multifunctional chaperone promoting all levels of SOD1 maturation”, Journal of Biological Chemistry, 294, 1956-1966 (2019). PMID: 30530491

24. J. Calvo, Lopez V. and G. Meloni*, “Non-coordinative metal selectivity bias in human metallothioneins metal- thiolate clusters”, Metallomics, 10, 1777-1791 (2018) (featured on Journal Issue back Cover). PMID: 30420986     
                                                                                 
23. E. Atrian-Blasco, A Santoro, D.L. Pountney, G. Meloni, C. Hureau and P. Faller, “Chemistry of mammalian metallothioneins and their interaction with amyloidogenic peptides and proteins”, Chemical Society Reviews, 46, 7683-7693 (2017). PMID: 29114657

22. Z. Chen, S. Boyd, J. Calvo, K. Murray, G. Mejia, C. Benjamin, R. Welch, D. Winkler, G. Meloni, S. D'Arcy, J. Gassensmith, "Fluorescent Functionalization Across Quaternary Structure in a Virus-Like Particle", ACS Bioconjugate Chemistry, 20, 2277-2283 (2017). PMID: 28787574

21. X. Zhen, B. Li, F. Hu, S. Yan, G. Meloni, H. Li, N. Shi "Crystal structure of the DNA-binding domain of Myelin-gene Regulatory Factor", Scientific Reports, 7, 6396 (2017). PMID: 28623291

20. M. Vasak*, G. Meloni* "Mammalian Metallothionein-3: New Functional and Structural Insights", International Journal of Molecular Sciences, 18, 1117-1131 (2017). PMID: 28538697

19. J. Calvo, H. Jung, G. Meloni* "Copper metallothioneins", IUBMB Life, 69, 236-245 (2017). PMID: 28296007

18. D. Mattle, L. Zhang, O. Sitsel, L.T. Pedersen, M.R. Monicelli, F. Tadini-Buoninsegni, P. Gourdon, D.C. Rees, P. Nissen, G. Meloni* “A sulfur-based transport pathway in Cu+-ATPases”, EMBO Rep., 16, 728-740 (2015) (*Corresponding Author). PMID: 25956886

17. O. Sitsel, C. Gronberg, H. Autzen, K. Wang, G. Meloni, P. Nissen, P. Gourdon. "Structure and function of Cu(I)- and Zn(II)-ATPases", Biochemistry, 54, 5673-5683 (2015). PMID: 26132333 

16. K.T. Wang°, O. Sitsel°, G. Meloni, H. E. Autzen, M. Andersson, T. Klymchuk, M. Nielsen, D.C. Rees, P. Nissen, P.  Gourdon, “Crystal structure and release mechanism of Zn2+-transporting P-Type ATPases”, Nature, 514, 518-522 (2014) (°contributed equally). PMID: 25132545

15. G. Meloni*, L. Zhang*, D.C. Rees* “A transmembrane Type-2-like Cu(II) site in the P1B-3-type ATPase CopB: implications for metal selectivity”, ACS Chemical Biology, 17, 116-121 (2014) (*Corresponding Author). PMID: 24144006

14. L. Zhang, J.T. Kaiser, G. Meloni, K.Y. Yang, T. Spatzal, S.L. Andrade, O. Einsle, J.B. Howard, D.C. Rees “The 16th Fe in Nitrogenase MoFe-Protein” Angewandte Chemie International Edition, 52, 10529-10532 (2013). PMID: 23963815

13. D. Mattle, O. Sitsel, H.E. Autzen, G. Meloni, P. Gourdon, P. Nissen, “On allosteric modulation of P-type Cu+-ATPases”, Journal of Molecular Biology, 425, 2299-2308 (2013). PMID: 23500486

12. G. Meloni*, A. Crameri, G. Fritz, P. Davies, D.R. Brown, P.M.H. Kroneck, M. Vašák*, “The Catalytic Redox Activity of Prion Protein–Cu(II) is Controlled by Metal Exchange with the Zn(II)–thiolate Clusters of Zn7Metallothionein-3”, ChemBioChem, 13, 1261-1265 (2012) (*Corresponding Author). PMID: 22615124

11. Y. Manso, J. Carrasco, G. Comes, G. Meloni, P. Adlard, A. I. Bush, M. Vašák, J. Hidalgo, “Characterization of the role of metallothionein-3 in an animal model of Alzheimer’s disease”, Cellular and Molecular Life Sciences, 69, 368-3700 (2012). PMID: 22722772

10. M. Vašák, G. Meloni, “Chemistry and Biology of mammalian metallothioneins”, Journal of Biological Inorganic Chemistry, 16, 1067-1078 (2011). PMID: 21647776

9. G. Meloni*, M. Vašák*, “Redox Activity of α-Synuclein-Cu is Silenced by Metallothionein-3”, Free Radicals Biology & Medicine, 50, 1471-1479 (2011) (*Corresponding Author). PMID: 21320589

8. J. Durand, G. Meloni, C. Talmard, M. Vašák, P. Faller, “Zinc release of Zn₇metallothionein-3 induces fibrillar type amyloid-β aggregates”, Metallomics,1, 741-744, (2010). PMID: 21072365

7. G. Meloni*, M. Vašák*, “Control of abnormal metal-protein interactions in neurodegenerative disorders by metallothionein-3”, CHIMIA, 63, 211-213 (2009) (*Corresponding Author). DOI: 10.2533/chimia.2009.211

6. G. Meloni°, T. Polanski°, O. Braun, and M. Vašák, “Effects of Zn2+, Ca2+, and Mg2+ on the Structure of Zn7Metallothionein-3: Evidence for an Additional Zinc Binding Site”, Biochemistry, 48, 5700-5707 (2009) (°contributed equally). PMID: 19425569

5. G. Meloni, V. Sonois, T. Delaine, L. Guilloreau, A. Gillet, J., Teissie, P. Faller, M. Vašák, “Metal swap between Zn7metallothionein-3 and amyloid-β-Cu protects against amyloid-β toxicity”, Nature Chemical Biology, 4, 366-372 (2008). PMID: 18454142

4. G. Meloni, P. Faller, M. Vašák, “Redox silencing of copper in neurodegenerative disorders: Reaction of Zn7metallothionein-3 with Cu(II) ions”, Journal of Biological Chemistry, 282, 16068-16078 (2007). PMID: 17389590

3. G. Meloni, K. Zovo, J. Kazantseva, P. Palumaa, M. Vašák, “Organization and assembly of metal-thiolate clusters in epithelium-specific metallothionein-4”, Journal of Biological Chemistry, 281, 14588-14595 (2006). PMID: 16556599

2. G. Meloni, M. Knipp, M. Vašák, “Detection of neuronal growth inhibitory factor (metallothionein-3) in polyacrylamide gels and by Western blot analysis”, Journal of Biochemical and Biophysical Methods, 29, 76-81 (2005). PMID: 15982745

1. M. Knipp°, G. Meloni°, B. Roschitzki, M. Vašák, “Zn7metallothionein-3 and the synaptic vesicle cycle: interaction of metallothionein-3 with the small GTPase Rab3A”, Biochemistry, 44, 3159-3165 (2005). (°contributed equally). PMID: 15736926

Book chapterS

1. M. Vašák*, G. Meloni* “Zinc Metallothionein-3 (Neuronal growth inhibitory factor)” Encyclopedia of Metalloproteins, (Ed. V. Uversky, R. H. Kretsinger, E. A. Permyakov), Springer, New York, USA, pp. 2486-2491, (2013) (*Corresponding Author).

2. M. Vašák, G. Meloni, “Metallothionein-3, Zinc, and Copper in the Central Nervous System”, in Metal Ions in Life Science Vol. 5, Metallothioneins and Related chelators, (Ed. A. Sigel, H. Sigel, and R. K. O. Sigel), The Royal Society of Chemistry, Cambridge, UK, pp. 319-351, (2009).

3. M. Vašák*, G. Meloni*, “Metallothionein Structure and Reactivity”, in Metallothioneins in Biochemistry and Pathology, (Ed. P. Zatta), World Scientific Publishing, Singapore, pp. 1-24 (2008) (*Corresponding Author).